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KMID : 0043320090320071045
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Archives of Pharmacal Research
2009 Volume.32 No. 7 p.1045 ~ p.1054
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Enhanced Transfection of Polyplexes Based on Pluronic-Polypropylenimine Dendrimer for Gene Transfer
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Sha Xian-Yi
Fang Xiao-Ling
Li Ya-Juan
Zhang Wei
Zhang Zhi-Wen
Jiang Ye
Tang Yuan-Jia
Hao Jun-Guo
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Abstract
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Third generation cationic dendritic polymeric polypropyleneimine (PPI) was modified by Pluronic P123 and investigated for gene delivery. The cytotoxicity of P123-PPI was evaluated by the MTT assay and shown to be much lower than that of PPI alone. P123-PPI and PPI can both condense plasmid DNA into nanoparticles with a size of approximately 100 nm and a zeta potential of about 15 mV at the N/P ratio 20:1. The nanoparticles can protect plasmid DNA from being digested by DNase I at a concentration of 0.4 U/μg DNA. The nanoparticles were resistant to dissociation induced by 50% fetal bovine serum and 75 μg/mL sodium heparin. The transfection efficiency of SPC-A1 cells using P123-PPI/DNA nanoparticles was much higher than the transfection utilizing PPI/DNA nanoparticles. The addition of free P123 during the preparation of P123-PPI/DNA nanoparticles could significantly enhance the transfection efficiency in the presence of 10% fetal bovine serum. Therefore, P123-PPI/DNA complex nanoparticles may be a safe, efficient and promising cationic conjugate for gene delivery.
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KEYWORD
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Pluronic, Polypropylenimine, Polyplex, Nanoparticles, Non-viral vector, Gene delivery system
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